하인두편평세포암 (FaDu) 세포에서 HomeoboxC6가 Sirt1 발현 조절을 통해 세포 증식 유도
HomeoboxC6 Promotes Cell Survival by Regulating the Sirt1 Expression in Hypopharyngeal Squamous Cell Carcinoma (FaDu) Cells
원옥튀띠엔, 정연수, 응웬 칸 톤, 안상건,
소속 상세정보
원옥튀띠엔 ( Tien Nguyen Ngoc Thuy ) -
정연수 ( Jeong Yun-Soo ) -
응웬 칸 톤 ( Nguyen Khanh Toa ) -
안상건 ( Ahn Sang-Gun ) -
Abstract
Patients with oral squamous cell carcinoma (OSCC) generally have an elevated expression of homeobox C6 (HOXC6) gene. We found that HOXC6 was the significantly upregulated gene in hypopharyngeal squamous cell carcinoma (FaDu) cells using RNA-seq analysis.
However, it remains unclear whether HOXC6 plays a role in tumor process mechanism. Our study aimed to explore the potential oncogenic role and the detailed molecular mechanism of HOXC6 in FaDu cells. In this study, Sirt1 was validated to be overexpressed in FaDu cells and associated with HOXC6 expression. Overexpression of HOXC6 promoted the cell colony formation, whereas inhibition of Sirt1 by Sirt1 inhibitor EX527 reduced cell proliferation/colony formation and migration, and induced apoptosis in HOXC6 overexpressed FaDu cells. Interestingly, mechanistic study showed that EX527 mediated Sirt1 suppression led to decreased HOXC6 expression and upregulation of Sirt1 significantly increased the expression of HOXC6. HOXC6 was shown to cooperate with Sirt1 to enhance cell survival. We propose that HOXC6 promotes cell growth/colony formation, and that the HOXC6 may be a progression of hypopharyngeal carcinoma by activating Sirt1 pathways.
키워드
HOXC6; Sirt1; HNCC; EX527
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